If it is, the sample dilution factor will be shown in the diagram as above the 12 in the erlenmeyer flask is the sample dilution factor. Dilution worksheet and problems biology libretexts. Dilution and concentration theory dilution and concentration theory sounds ominous but you use dilutions and concentrations fairly frequently outside of your scientific life. Sample dilution factor sdf a sample is often diluted prior to doing the serial dilutions. Determine the concentration or dilution factor for the flasks and plates. The resulting serial dilution series has a dilution factor of approximately 1. Answers serial dilutions practice worksheet biol 307 studocu. Dilution is the addition of solvent, which decreases the. All materials were written for and are currently used.
Dilution displaying top 8 worksheets found for this concept some of the worksheets for this concept are dilutions work, dilutions work w 329, dilution name chem work 15 5, dilutions work, dilution work answers, chemistry dilution practice, dilutions work name key, solutions work 2 molarity and dilution problems answers. Displaying all worksheets related to serial dilutions. In the 1st of your small containers, place 10 drops of food coloring. Once you find your worksheet, click on popout icon or print icon to worksheet to print or download. Answers serial dilutions practice worksheet biol 307. These include quantifying the number of bacteria in a sample using plate counts and the development of standard curves for quantitative colorimetric, radiometric, and enzymatic assays. I made this for my as biology students who dont take chemistry and needed some practice with serial dilutions before jumping straight into a full practical. Dilution techniques and pipetting biology libretexts. We worked through the first example together on the board and the students did the dilution using coloured water. Some of the worksheets displayed are chemistry dilution practice, dilutions work, dilutions work, dilutions work w 329, dilution work answers, extra molarity problems for practice, working dilution problems, molarity and serial dilutions teacher handout. How to work microbiology dilution problems sciencing. Remember, keep going until youre very comfortable with the calculations. Showing top 8 worksheets in the category dilution problems.
Molarity and serial dilutions teacher handout module overview one of the most important skills in science is to calculate dilutions. Serial dilution many procedures performed in modern biology and chemistry laboratories require sets of solutions that cover a range of concentration s. To determine the concentration you multiply the number of colonies by the inverse of the dilution factor in flask b it was 104. Calculating final dilution in a given serial dilution problem.
Molarity practice problems this chemistry video tutorial explains how to solve common molarity problems. Once again, heres an applet to practice finding the total dilution scheme, regardless of how the dilution scheme is expressed as directions, as fractions, or as decimals. Thus, a series of 5, one to ten dilutions equals a one to one hundred thousand dilution. Jun 01, 20 the resulting serial dilution series has a dilution factor of approximately 1. Serial dilution protocol pdf it is a method of diluting a stock solution where concentration decreases by the same quantity in each successive step. In this dilutions worksheet, students calculate molarity of solutions and practice figuring out the volume of concentrate needed for a specific dilution of solutions.
Most specimens have high enough numbers of microorganisms that the specimen has to be serially diluted to quantitate effectively. A dilution of a bacterial suspension was prepared by adding 100 lambdas of the suspension to 9. In the lab, correct dilutions of solutions are critical to performing accurate science. Dilution problems worksheet worksheet fun and printable. You will make several dilutions of a bacterial stock culture. There are three formulas used to work microbiology dilution problems. Here you will find all we have for dilution problems worksheet. This worksheet defines stock solution, serial dilution and provides 3 practice problems. Dilution is a decrease in a solutions concentration, whereas concentration is an increase in a solutions concentration. Dilutions worksheet w 329 everett community college. Serial dilutions practice problems san diego miramar college. Aug, 1995 a dilution of a bacterial suspension was prepared by adding 100 lambdas of the suspension to 9. To make a dilution series, use the following formulas. So you multiply each successive dilution by the dilution factor.
In medicine, these skills are necessary to ensuring proper medicine dosage and treatment. Remember that serial dilutions are always made by taking a set quantity of the initial dilution and adding it successively to tubes with the same volume. Well, that formula, c i v i c f v f, is a perfectly good one to use for most types of dilution problems, as long as the units on both sides of the equation match. For example, when you are making orange juice from a frozen concentrate, you mix 1 can of concentrate with 3 cans of water. Dilution factor practice problem to dilute a bacterial culture, 500. Calculating final dilution in a given serial dilution problem skills practiced. Pltw, mi unit 1 serial dilution practice quiz quizizz. Dilutions are useful in science when making solutions or growing an acceptable number of bacterial colonies to count. Lab math solutions, dilutions, concentrations and molarity. Color the first circle the color of the container with food coloring or describe the color in the circle. A dilution is the mixing of a small accurately measured sample with a large volume of diluent usually sterile water or saline solution 1.
It is a common practice to determine bacterial counts for both liquid and solid specimenssuspensions of e. Click on popout icon or print icon to worksheet to. Free gamified quizzes on every subject that students play in class and at home. As understood, deed does not suggest that you have wonderful points. Serial dilution displaying top 8 worksheets found for this concept some of the worksheets for this concept are dilutions work, dilutions work w 329, molarity and serial dilutions teacher handout, dilutions work, laboratory math ii solutions and dilutions, lab math solutions dilutions concentrations and molarity, well what will we drink, dilutions work name key. Serial dilution practice problem set science primer.
Sample math problems for microbiology david fankhauser. Dilutions worksheet 1 if i add 25 ml of water to 125 ml of a 0. Then 1 ml from flask a is transferred to flask b and then 1 ml from flask b is transferred to flask c. To find the overall or total dilution factor, simply multiply the dilution factors for each step. Key is licensed under a creative commons attributionnoncommercialsharealike 4. The most common type of dilutions done in chemistry lab involve taking a sample of a solution of known volume and concentration and diluting it to some larger volume. Often, a worker will need to change the concentration of a solution by changing the amount of solvent. Defining key concepts ensure that you can accurately define main phrases, such as solution and molarity. The answers i sure hope you tried to answer the problems before you looked here. Determine how many moles of solute and liters of solvent you have for each solution you are. Easy speed math they dont teach you in school part 1 addition duration.
This is just one of the solutions for you to be successful. In a serial dilution, the final total dilution is a product of each individual dilution in the series. Dilutions and concentrations introductory chemistry. Reading comprehension ensure that you draw the most important information from the properties of serial dilution lesson. Reading comprehension ensure that you draw the most. Below is an applet to practice finding dilution factors, and also to determine how much water to add to achieve a given dilution. The purpose can be determination of bacterial, fungal, or viral counts indirectly. In each of the rest of your small containers, place 9 drops of water. In fact, it relies on the principle used in the above calculations.
The following problem sets test your ability to calculate dilution factors and concentrationsdilution factor calculationconcentration of a dilution. If students are not familiar with operating pipettes, the pre lab serial dilutions practice exercise is recommended. Good practice for those who have never used a syringe too. Working dilution problems it is a common practice to determine microbial counts for both liquid and solid specimenssuspensions of e. This type of dilution series is referred to as a serial dilution. Worksheets are chemistry dilution practice, dilutions work, dilutions work, dilutions work w 329, dilution work answers, extra molarity problems for practice, working dilution problems, molarity and serial dilutions teacher handout. A serial dilution is a series of simple dilutions which amplifies the dilution factor quickly. The following is a stepbystep procedure to working dilution problems, and includes some practice problems at the end.
If the sample remains undiluted, use 11 as the sample dilution factor. Dilution of solutions worksheet 1 free download dilution of solutions worksheet. Dilutions worksheet socorro independent school district. Some of the worksheets displayed are dilutions work, chemistry dilution practice, dilutions work w 329, dilutions work, making dilutions work, molarity molarity, chem1001 work 6 concentration model 1 concentration, concentration work w 328. Dilutions worksheet solutions 1 if i add 25 ml of water to 125 ml of a 0. A set of serial dilutions is made, a sample of each is placed into a liquefied agar medium, and the medium poured into a petri dish. A serial dilution is any dilution in which the concentration decreases by the same factor in each successive step in serial dilutions, you multiply the dilution factors for each step. This is a chemistry tutorial that covers dilution problems, including examples of how to calculate the new concentration of a diluted solution, and how to calculate the volume of a concentrated. Displaying all worksheets related to dilution problems. Here you will find all we have for dilution worksheet. The following protocol is a stepbystep procedure to working dilution problems, and includes some practice problems at the end. Concentrations and dilutions answer key kiddy math.
The important thing to remember is that the amount of solute doesnt change during the dilution process, only the amount of solvent and. From the original culture 1 ml of broth was transferred to flask a which contains 99 ml of sterile water. A dilution series is a succession of step dilutions, each with the same dilution factor, where the diluted material of the previous step is used to make the subsequent dilution. Serial dilutions practice problems refer to the below image. This worksheet and quiz will let you practice the following skills. Concentrations and dilutions answer key displaying top 8 worksheets found for this concept some of the worksheets for this concept are dilutions work, dilutions work, dilutions work name key, dilutions work w 329, concentrations and dilutions, molarity and serial dilutions teacher handout, laboratory math ii solutions and dilutions. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Most specimens have high enough numbers of microorganisms that. This worksheet and quiz let you practice the following skills. Dilutions worksheet awesome science teacher resources. Dilutions and concentrations introductory chemistry 1st. Worksheets are dilutions work, dilutions work w 329, molarity and serial dilutions teacher handout, dilutions work name key, laboratory math ii solutions and dilutions, making dilutions work, lab math solutions dilutions concentrations and molarity, lab basic skills dilutions micropipettes and. The exercise will allow students to learn how to pipette, dilute samples and calculate the concentration of their samples. Dilutions worksheet w 329 everett community college student support services program 1 if 45 ml of water are added to 250 ml of a 0.
420 1211 148 1205 181 783 98 1277 1416 303 1116 818 155 1402 991 532 1367 601 300 149 1227 1238 346 861 664 1239 348 373 1466